Regulatory

Part:BBa_K3385006:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-12)


Ptef


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is well known and is known to work and can be cloned using several cloning methods, such as USER and Gibson.

Source

Obtained as part of the CRISPR cloning vector pFC330[1]. It originates from A. nidulans.

pFC330 was a gift from Uffe Mortensen (Addgene plasmid # 87842; http://n2t.net/addgene:87842; RRID:Addgene_87842)

References

[1] A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi. Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH. PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015. PONE-D-15-11561 [pii] PubMed 26177455